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Vectors & Plasmids

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BHL9 Barley High Lysine plasmid - Pioneer (posted 2/1/11)

Clemente website for protocols (updated 2/5/15)

CodA plasmids (posted 2/1/11)

nptII - GFP - Altpeter

Altpeter, F. 2006. Perennial Ryegrass (Lolium perenne L.). In: Methods in Molecular Biology, vol. 344: Agrobacterium Protocols, 2/e, volume 2 (Wang, K., ed.) Humana Press Inc., Totowa, NJ, pp. 55-64. (posted 2/1/11)

NcoI digest of pYF132 (postd 2/1/11)

pYF132 - ubi-nptII-T35S - UbiI-sGFPS65T-Tnos (posted 2/1/11)

pAct1IHPT-4 Map - Cho (posted 2/1/11)

pAHC17 map - Christensen (posted 2/1/11)

pCAMBIA1300

pCAMBIA-1300 complete sequence (posted 2/1/11)

pCAMBIA1300 map (posted 2/1/11)

pCAMBIA1302

pCAMBIA1302 sequence (posted 2/1/11)

pCAMBIA2300

pCAMBIA 2300 GenBank sequence (posted 2/1/11)

pGreen + pSOUP plasmid system: Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean. S. and Mullineaux, P.M. 2000. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Molecular Biology 42: 819-832. (posted 2/1/11)

pPZP111 - Maliga

pPZP111 sequence (posted 2/1/11)

Restriction map pPZP111 (posted 2/1/11)

pPZP201, 212, pTN290, pPZP201-PMI-GFP (posted 2/1/11)

pTF101 - Wang

pTF101 components (posted 2/1/11)

pTF101.1 map (posted 2/1/11)

pTM290 - Miller

pTN290 - Clemente

Clemente comment on NcoI sites (posted 2/1/11)

Gel pTN290 digests NcoI NotI SmaI (posted 2/1/11)

pFF19 - source of 35S promoter terminator (posted 2/1/11)

pPTN290 Clemente map (posted 2/1/11)

pUC18 19 MCS sequence

TRX NTR AcTPase hpt Bombardment Constructs (posted 2/1/11)

YFP, GFP maize optimized -Clemente

Accession numbers for maize-optimized GFP (posted 2/1/11)

pUC18-maize-GFP pUC57-maize-YFP-maize Clemente (posted 2/1/11)

Clemente Protocol (posted 4/19/10)